Upcoming Webinar - May 22
"Getting Started with AFM in Biology – It's Easier Than You Think"
Click on either time to register:
You may be a biologist new to the AFM or an AFM expert starting to study biology. When you first start out, using an AFM for biological applications can seem overwhelming. Although there are challenges for successful AFM in biology, we’ll show you it’s easier than you think!
Sample prep is a critical part of successful Bio-AFM. There are some basic principles that help insure success. However, life is complex and so are biological samples – with variations as large as the number of researchers. Thus, at the same time, you will need to be flexible – your samples may require a prep that is tweaked and tailored a bit to optimize your results. Working in liquid adds another challenge. In addition, the choice of measurement mode may not be obvious. Do I want to use tapping mode? Force curves? Contact Mode? Finally, there is a long list of commercial cantilevers available and choosing the best one can be like looking for a needle in a haystack. In this webinar we will present four case studies of a few typical biological samples:
1. Imaging DNA in liquid – including routine helix resolution
2. Imaging living cells in liquid
3. Measuring Young’s modulus of living cells
4. Unfolding forces in Titin
In each case we will discuss sample prep, lever and measurement mode choice and follow up with data interpretation and cautionary examples of experimental artifacts. The goal of this webinar is to give you the confidence to repeat these experiments yourself and then extending them to fit your own research.
About your lecturer
Irene Revenko is one of the world’s leading experts in Bio AFM. She is a staff scientist at Asylum Research and has over 19 years of AFM experience. She initiated the first bio-classes at Asylum Research in 2002 and since then has taken many students from their first AFM measurements through cutting edge results.
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Nanomechanics: “Introduction and Innovations in High Speed Quantitative Nanomechanical Imaging”
This presentation will begin with a survey of the mechanical properties that can be investigated with the wide array of both old and new nanoscale property mapping techniques available to materials scientists. We will then introduce two new techniques for nanomechanical studies that allow unambiguous interpretation of material properties: AM-FM and Loss Tangent. Amplitude-modulated (AM) atomic force microscopy, also known as tapping mode, is a proven, reliable and gentle imaging method with wide spread applications. Previously, the contrast in tapping mode has been difficult to quantify. The new AM-FM imaging technique combines the features and benefits of normal tapping mode with the quantitative, high sensitivity of Frequency Modulation (FM) mode. Loss Tangent imaging is another recently introduced quantitative technique that recasts the interpretation of phase imaging into one term that includes both the dissipated and stored energy of the tip sample interaction. These techniques allow high speed, low force imaging in tapping mode while providing quantitative Elasticity and Loss Tangent images.
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"Smaller and Quieter: Ultra-High Resolution AFM Imaging"
Miniaturization of cantilevers for Atomic Force Microscopy has increased their resonant frequencies and decreased their thermal noise, allowing faster, lower noise measurements. When used in the extremely low-noise Cypher AFM, these levers have enabled significant improvements in imaging resolution in air and especially in liquids. On crystals, individual atomic point defects can now be routinely resolved and this higher resolution also extends to biological samples. Examples shown include the movement of individual point defects in bacteriorhodopsin, atomic point defects in calcite, and resolution of the double-helix structure of DNA in solution.
This first webinar in our 2012 Webinar Series was presented by AFM pioneer, inventor and Asylum Research co-founder, Dr. Jason Cleveland.
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To receive a copy of the recorded webinar, please email us.