Closed Fluid Cell

Accessory for the MFP-3D™ AFM

The Closed Fluid Cell allows fluid imaging in a completely sealed and enclosed environment.

Completely Sealed Design
The Closed Fluid Cell (CCELL) is designed to hold gases or liquids either statically or as part of a flow-through set-up in a completely enclosed and sealed cell. The CCELL is sealed by means of a flexible membrane which mounts to the MFP-3D cantilever holder. The membrane allows the tip to move freely relative to the sample, thereby allowing for distortion-free scans. The CCELL also employs replaceable O-rings that maintain seals up to 2.5psi of water pressure to allow pressurized fluid exchange. The CCELL can also be operated in an open configuration where a soft seal membrane is used to minimize evaporation and contamination.

Ten Access Ports for Fluid or Gas Exchange
The fluid cell contains four 1/16" and six 0.036" diameter ports for fluid or gas exchange via syringe injection, gravity feed, or pump systems.

Operation on the MFP-3D
The fluid cell magnetically attaches to the MFP-3D scanner and supports both bottom view and top view optics. Each system is tested for six feet (2m) of water pressure (2.5psi), which exceeds manual syringe pressure as well as most gravity feed system setups.

Accommodates a Variety of Sample Mounts
Each cell consists of a PEEK™ base that holds a 35mm diameter by 1mm thick glass disc for sample mounting. This glass disc can be replaced with special mounts that can hold 12mm or 25mm circular cover slips for high NA inverted optical microscopy.

Easy Cleaning Alleviates Contamination
All of the CCELL components can be easily removed and cleaned. The only materials that contact the fluid are PEEK, glass, silicone, FKM (Viton® equivalent O-ring material) and the cantilever holder. The entire CCELL (excluding the cantilever holder) can be sterilized in an autoclave.

A Suite of Fluid Cell Accessories
The Closed Fluid Cell is one in a suite of environmental accessories that can be used with the MFP-3D to meet your experimental needs. Please see the separate data sheets on these options for more information.

Fluid Cell Lite
Fluid Cell Lite is an economical choice for fluid imaging with a portless fluid dish and an evaporation shield that prevents accidental leakage.

BioHeater™
The BioHeater is the Closed Fluid Cell with added heating capability, allowing heating of samples in liquids to 80°C in a sealed and enclosed environment. Requires Environmental Controller (sold separately).

Petri Dish Holder and Heater
The Petri Dish Holder allows cell samples to be cultured and imaged in Petri dishes. The Petri Dish Heater adds additional sample heating functionality from ambient to 45°C. Requires Environmental Controller (sold separately).

Specifications

Closed Fluid Cell – Model CCELL

The Closed Fluid Cell is packaged in a convenient storage kit and includes the following items for installation and imaging:

* Not pictured

Completely sealed cell

Gas flow through

Top and bottom optical access

Maximum pressure 2.5psi

Sample size
- 25mm maximum; 2mm thickness
- 10mm sample travel within cell when open
- 1-3mm sample travel within cell when closed and scanning, dependent upon tightness of clamp.

Fluid volume
- 5ml before engage
- 1.5ml-2.55ml after engage, clamped and sealed

Access ports (not pictured)

Cleaning
- Easy and complete disassembly
- Autoclavable

Materials

MFP-3D and BioHeater are trademarks of Asylum Research. Other trademarks are those of their respective owners. Specifications and kit items subject to change without notice.

Download Closed Fluid Cell PDF

High Voltage Compatible

Closed Fluid Cell mounted to the cantilever holder on the MFP-3D head as it is being lowered into scanning position.

Schematic of Closed Fluid Cell assembly.