BioHeater™ Closed Fluid Cell
Accessory for the MFP-3D™ AFM
The BioHeater, based on our Closed Fluid Cell, is a temperature controlled sample stage for the MFP-3D AFM for heating samples in liquids from ambient to 80°C. It is ideal for many applications such as protein conformational changes, enzymatic activity, lipid phase transitions and others.
The BioHeater consists of a closed fluid cell with an immersed heating element which symmetrically heats the surrounding fluid. The fluid cell has a total of ten ports. Seven are available for liquid and gas exchange and electrical access to the sample. The remaining three are used by the heating element.
The fluid cell can be operated in either an open configuration where the membrane cover acts as an evaporation shield, or with a clamp for a completely sealed and controlled environment. It supports samples up to 2mm thick. A stainless steel temperature probe extends near the sample for closed loop control within 0.1°C. Materials in contact with the liquid are glass, cantilever holder materials, silicone rubber, FKM (Viton® equivalent O-rings), stainless steel (temperature probe), PEEK, and FEP Teflon® coating (on the heating element). The BioHeater fluid cell can be completely disassembled for cleaning and most parts can be sonicated and autoclaved.
Closed Loop Accuracy with the Environmental Controller
BioHeater – Model BIOHEAT
The BioHeater includes the Heating Stage (which can be reconfigured as a Closed Fluid Cell) and Accessory Kit. Requires the Environmental Controller (purchased separately):
* Not pictured
Compatible with all high NA objectives (0.17mm cover slip bottom recommended)
Liquid flow through
Top and bottom optical access
Maximum pressure 2.5psi
Access ports (not pictured)
Environmental Controller – Model ENVIRO
• Closed loop operation
MFP-3D, SmartStart and BioHeater are trademarks of Asylum Research. Other trademarks are those of their respective owners. Specifications and kit items subject to change without notice.
The BioHeater allows thermally controlled experiments in liquids in a totally sealed environment.
Temperature vs. time graph which includes temperature setpoint (blue line), sensor (sample) temperature (black line), and heater output (red line).
Schematic of BioHeater assembly.