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AFM image overlaid with the epifluorescence image of a patterned cardiac myocyte.

A rat cardiac myocyte was cultured on a micro-contact-printed square island of
extra-cellular matrix (ECM) protein on a PDMS substrate. The cell was fixed and stained for
actin (green), sarcomeric alpha-actinin (red) and DNA (blue). After fixation,
the cell was scanned by AFM. Merged topography and fluorescent images reveal
that the actin/alpha-actinin myofibrils and the nucleus are responsible for specific
AFM topography. The 92µm image was taken by the Disease Biophysics
Group at the School of Engineering and Applied Sciences, Harvard University,
headed by Prof. Kevin Kit Parker.

Image courtesy of K. Parker and N. Geisse, Harvard University.

Control of Myocyte Remodeling In Vitro with Engineered Substrates
Geisse NA, Sheehy SP, Parker KK
In Vitro Cell Dev Biol Anim. 2009. 45(7):343-50.

 


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