Asylum Research

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Fixed Fibroblast Cells

Fixed fibroblast cells (MRC-5 cell line) acquired in contact mode using the
200µm long Olympus TR400PB cantilever. The cells were grown in a Falcon
petri dish and fixed with 4% glutaraldehyde. They were imaged with the new Petri Dish
Holder Accessory on the MFP-3D-BIO. Brightfield illumination was initially used to locate
cells of interest and accurate position the cantilever over the cells (a). AFM Deflection
image (b) and 3D rendered topographic image using Argyle (c). 70µm AFM scan size.



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